These are the important Questions asked from Biotechnology Chapter in JKBOSE/other State Board Class 12th Class Examinations

Note: Only Short & very short answer type Questions are asked from this chapter

Q1. Define Cloning?

Q2. Explain briefly:-

(i) PCR (ii) Restriction enzymes & DNA (iii) Chitinase

Q3. Diagrammatically represent the experimental step in cloning and express as human gene into a bacterium like E-Coli?

Q4. What is Human Insulin? Give It's Uses?

Q5. What are Biosafety issues?

Q6. Define Plasmid?

Q7. How are restriction enzymes helpful in recombinant DNA technology?

Q8. Define Clone?

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JKBOSE 12th Class Biology Papers of last 10 years | Important Questions

Q9. What is Gene therapy? Give significance?

Q10. Mention two applications of cloning.

Q11. Name and explain the various steps involved in Recombinant-DNA technology.

.Q12. Describe in detail the method of PCR technique and its applications.

Q13. Explain in brief Southern blotting technique and its applications.

Q14. Define Genomics and discuss Human Genome Project.

Q15. Describe the role of CaCl2 in the preparation of competent cell.

Q16. Describe briefly the following:

(a) Origin of replication (b) Bioreactors (c) Downstream processing

Q17. What are Cry proteins? Name an organism that produce it.

Q18. What are transgenic bacteria? Illustrate using any one example?

Q19. Distinguish between:

(a) Plasmid DNA and Chromosomal DNA

(b) RNA and DNA

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